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The term?whole liver? refers to the entire liver in addition to the local areas of tissue injury or necrosis. In order to understand how the healthy liver responds to injury and recover from ALD, an interdisciplinary approach combining cell signaling and molecular biology, molecular physiology and biophysics, and novel in vivo imaging is required. The overall goal of this project is to develop a systems approach to understanding the basic processes of liver regeneration that will significantly improve our understanding of normal liver cell behavior in response to injury and recovery from injury. The hypothesis is that the liver responds by a complex combination of cell death and cell proliferation. For this to be an effective model, the biochemical and physiological changes in the liver must be accurately reflected by the microscopic changes in the liver. In order to determine the impact of chemical/physical injury to the liver, we will use a combination of novel optical imaging and molecular probes that detect key aspects of regeneration, allowing us to determine the relationship between biochemical and macroscopic in vivo imaging. Specifically, we will use a combination of biochemical, optical, and molecular tools to: 1. determine how micron-scale features of structural tissue remodeling affect the ability of cells to respond to chemical/physical liver injury; 2. determine how damage to the membrane affects the ability of cells to respond to liver injury; and 3. determine the accuracy of in vivo optical imaging of liver injury in the mouse to predict changes in biochemical measures of liver injury/regeneration. The project will interact with other grantees at Duke University and medical centers at UNC-CH and UNC-CH-CH to ensure that the results of this project are applicable to the disease of ALD.Familial cerebral angiitis in Rasmussen's encephalitis: clinical and pathological features. Rasmussen's encephalitis is a rare intractable epilepsy of early childhood. Here we describe a patient with a family history of Rasmussen's encephalitis. Magnetic resonance imaging of the brain in the patient demonstrated multiple cerebral microbleeds. With immunosuppressive treatment she showed remission of epilepsy and improved cognitive function. 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